Rabbit Polyclonal Anti-HECTD1 Antibody against Human HECT domain containing E3 ubiquitin protein ligase 1. Validated for Immunofluorescence and ImmunohistochemistryAnti-HECTD1 Antibody (HPA002929) - Atlas AntibodiesWas this helpful?People also askDo you need a secondary antibody raised in rabbit?Do you need a secondary antibody ...
Anti-HECTD1 antibody produced in rabbit is polyclonal, unconjugated and is suitable for immunofluorescence, immunohistochemistry Are there alternatives to polyclonal anti - rabbit IgG secondary antibodies?Are there alternatives to polyclonal anti - rabbit IgG secondary antibodies?Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Enhanced Chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)mouse anti-rabbit IgG-HRP Secondary Antibody SCBT anti hectd1 rabbit primary antibodies
Goat Anti-rabbit IgG (H+L) secondary antibody, 10 nm Colloidal Gold Conjugate, for detection, localization, distribution and quantification of target proteins at an ultrastructural level via indirect immunogold staining in IHC-P, IHC-F, ICC, or EM. Reagent Type. Colloidal gold conjugated secondary antibody. Conjugate. Colloidal gold, 10 nm. Host.
Secondary antibodies bind to the heavy chains of primary antibodies, so that they dont interfere with the primary antibody binding to the antigen. This secondary antibody is made in a species that is different than both those of the primary antibody or the specimen. This minimizes non-specific binding that leads to false positives and high anti hectd1 rabbit primary antibodies4.5/5(8)BV421,Goat,Anti-Rabbit,IgG,Polyclonal,RUO - 565014 BD anti hectd1 rabbit primary antibodiesBD Horizon BV421 Goat Anti-Rabbit IgG is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rabbit IgG primary antibodies. It reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. BV421 Anti-Rabbit IgG has minimal crossreactivity with human, mouse, or rat serum proteins.Anti Mouse/Rabbit IgG (H+L), made in horse, BiotinylatedThis biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue. Features Recognizes both heavy and light chains (H+L) Biotinylated to ensure the maximum degree of labeling without compromising the specificity anti hectd1 rabbit primary antibodies
Anti-HECT domain E3 ubiquitin protein ligase 1 antibodies are available from several suppliers. In humans, this protein is encoded by the gene HECTD1. The protein may also be known as EULIR, E3 ubiquitin-protein ligase HECTD1, and E3 ligase for inhibin receptor.Anti-HECTD1 Antibodies Invitrogen10 antibodies to HECTD1 and validated for use in 5 applications (Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence)Anti-HECTD1 Rabbit Polyclonal Antibody VWRRabbit Polyclonal HECTD1 Antibody. Tested Applications Western Blot, Immunoprecipitation. Tested Reactivity Human.<B>IgGy Antibody Selector</B> Quickly search hundreds of thousands of antibodies available for purchase from VWR by selecting common antibody features like antigen symbol and name, reactivity, clonality, conjugation, host, and other key factors.
Predicted band size 290 kDa. Exposure time 3 minutes. Immunoprecipitation - Anti-HECTD1 antibody (ab101992) ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection Chemiluminescence with exposure time of 3 minutes.Anti-HECTD1 antibody (ab101993) AbcamImmunoprecipitation - Anti-HECTD1 antibody (ab101993) ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101993 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection Chemiluminescence with exposure time of 3 minutesAnti-HECTD1 antibody produced in rabbit Prestige anti hectd1 rabbit primary antibodiesAnti-HECTD1 antibody produced in rabbit Prestige Antibodies &Powered by Atlas Antibodies, affinity isolated antibody Synonym KIAA1131 Human Protein Atlas Number HPA054461 Human Protein Atlas - View RNA & protein expression data of this target
The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA of mouse IgG. The plate was Anti-Rabbit Antibody Products BiocompareGoat Anti-Rabbit AF488 is a Great Secondary Antibody for Immunofluorescence. (4) Write a Review. Thermo Fisher Scientific. When a rabbit-derived primary antibody is used to stain an antigen of interest, an anti-rabbit secondary antibody is necessary to visualize the rabbit anti-mouse signal.Anti-p21 Rabbit Monoclonal Antibody (M00145-3) BosterbioThe anti-p21 Rabbit Monoclonal antibody (M00145-3) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody
Primary Antibody Dilution Buffer 1X TBST with 5% BSA or 5% nonfat dry milk as indicated on primary antibody datasheet; for 20 ml, add 1.0 g BSA or nonfat dry milk to 20 ml 1X TBST and mix well. Secondary Antibody Conjugated to HRP Anti-rabbit ; anti-mouse . Brand Sigma-AldrichAnti-HECTD1 Antibody (HPA002929) - Atlas AntibodiesRabbit Polyclonal Anti-HECTD1 Antibody against Human HECT domain containing E3 ubiquitin protein ligase 1. Validated for Immunofluorescence and ImmunohistochemistryCan I use two secondary antibodies? One is anti-mouse anti hectd1 rabbit primary antibodiesFor example, if the primary antibody is raised in mouse, an anti-mouse secondary antibody should be used. If it is raised in rabbit, an anti-rabbit secondary antibody should be used.
The Anti-Rabbit IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all rabbit IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted)is supplied as a Donkey anti-Rabbit Antibodies InvitrogenAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence).GFP (D5.1) Rabbit mAb Cell Signaling TechnologyIncubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:10001:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Proceed with detection (Section D).
Primary Antibody Dilution Buffer 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well. Biotinylated Protein Ladder Detection Pack . Blue Prestained Protein Marker, Broad Range (11-250 kDa) . Blotting Membrane and Paper This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.HECTD1 Antibody 20605-1-AP ProteintechHECTD1 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse samples. Cat.No. 20605-1-AP. KD/KO Validated.HECTD1 Antibody Bethyl Laboratories, Inc.Unconjugated Whole IgG Rabbit anti-HECTD1 Antibody, Affinity Purified suitable for IP applications. Visit Bethyl for all your antibody needs. Bethyl is now a part of Fortis Life Sciences.
Jan 05, 2017Primary antibody diluted in PBS was added to the coverslips and incubated at 4 °C for overnight. anti hectd1 rabbit primary antibodies 500,MBL international corporation),Rabbit anti-IQGAP1 (H-109) (1 800, Santa Cruz Biotech, Dallas, USA), Rabbit anti-HECTD1(M03), clone 1E10 (1 100, Abnova, Taipei, Taiwan), Mouse anti-Giantin (1:1000, a gift from Prof. Martin Spiess anti hectd1 rabbit primary antibodiesHECTD1 controls the protein level of IQGAP1 to regulate anti hectd1 rabbit primary antibodiesTarget proteins were detected by western blot by using specific antibodies. Antibodies were used as Rabbit anti-HECTD1 (M03), clone 1E10 (1:1000, Abnova, Taipei, Taiwan), Rabbit anti-PIP5K1A (1:1000, Cell Signaling, Danvers, USA), Rabbit anti--Catenin (D10A8) (1:1000, Cell HECTD1 regulates the expression of SNAIL Implications the anti-HECTD1 (M03), clone 1E10 primary antibody (cat. no. H00025831-M03) was purchased from Abnova Corp. The FITC-conjugated and Alexa Fluor 546-conjugated anti-rabbit (A-11035) or anti-mouse (A-11003) secondary antibodies were purchased from Invitrogen; Thermo Fisher Scientific, Inc. and the horseradish peroxidase
The membranes were incubated with the following primary antibodies at 4C overnight Anti-ERK1/2 (1:200), anti-phospho-ERK1/2 (1:1,000), anti-HECTD1 (1:200) and anti-GAPDH (1:100,000). Following primary antibody incubation, the membranes were incubated with HRP-conjugated secondary antibodies (1:5,000) for 1 h at room temperature.Hsp90 alpha Recombinant Rabbit Monoclonal Antibody Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Images of Anti Hectd1 Rabbit Primary antibodies imagesHECTD1 Antibody (NBP1-49925) Novus BiologicalsRabbit Polyclonal Anti-HECTD1 Antibody. Validated WB, IP. Tested Reactivity Human, Mouse. 100% Guaranteed.
Mar 30, 2021Cells were fixed with 4% paraformaldehyde and then permeabilized with 0.3% Triton X-100 in PBS. After blocked with 10% normal goat serum in 0.3% Triton X-100, cells were incubated with mouse anti-GFAP antibodies (1:800; Sigma-Aldrich, #G3893) and/or rabbit anti-HECTD1 (1:200; Proteintech Group, #20,6051-AP) overnight at 4 °C.Is there a way double-color immunofluorescence staining anti hectd1 rabbit primary antibodiesThis does not solve your problem of having 2 rabbit primary antibodies, but again, the primary antibodies can be coupled to the oligonucleotide sequences directly, rending the use of a secondary anti hectd1 rabbit primary antibodiesLeptin Up-Regulates HECTD1 to Promote In this study, we investigate the regulation of the HECTD1-PIPKI90 pathway by leptin and examine the role of this pathway in regulating leptin-stimulated breast cancer cell migration and invasion. Materls and MethodsIa ragentse Anti-PIPKI90 polyclonal antibody was from Epitomics. Anti-HECTD1 rabbit polyclonal antibody was custom made by Syd anti hectd1 rabbit primary antibodies
The potential immunogenicity of acute lymphoblastic leukemia of the T cell (TALL), a small subgroup of childhood leukemia with increased risk for treatment failure and early relapse, was addressed b anti hectd1 rabbit primary antibodiesPATHWAY anti-HER-2/neu (4B5) Rabbit Monoclonal Primary anti hectd1 rabbit primary antibodiesThis antibody is intended for in vitro diagnostic use. Ventana Medical Systems, Inc.'s (Ventana) PATHWAY anti-HER-2/neu (4B5) Rabbit Monoclonal Primary Antibody (PATHWAY HER2 (4B5)) is a rabbit monoclonal antibody intended for laboratory use for the semi-quantitative detection of HER2 antigen in sections of formalin-fixed, paraffin-embedded normal and neoplastic tissue on a VENTANA Primary Antibody (Rabbit Anti-Chicken Polyclonal Antibody anti hectd1 rabbit primary antibodiesDescription Primary Antibody (rabbit anti-chicken polyclonal antibody), 25µg, is for use with the ELISA Immuno Explorer Kit (#166-2400EDU). The product is lyophilized for shipping and long-term storage. Primary Antibody is included in the ELISA Kit Reagent Refill Package (#166-2401EDU).
anti-HECTD1 antibody (HECT Domain Containing 1) Primary Antibody HECTD1 Reactivity Human, Mouse ELISA, IF, IHC, IP, WB Host Rabbit Polyclonal unconjugatedRabbit IgG (Control Antibody) - Vector LabsThe term irrelevant refers to a primary antibody of the same isotype as the specific primary antibody (i.e. mouse IgG) and applied at the same concentration, that is known not to bind to a target in the tissue specimen. An example would be an anti-cytokeratin antibody on smooth muscle tissue.Rabbit IgG (H&L) Secondary Antibody Peroxidase Goat anti-Rabbit IgG (H&L) Antibody Conjugated to Horseradish Peroxidase (HRP) is designed for chemiluminescent detection. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is anti hectd1 rabbit primary antibodies
Was this helpful?People also askDo you need a secondary antibody raised in rabbit?Do you need a secondary antibody raised in rabbit?For example, if you use a primary antibody raised in rabbit, you will need an anti-rabbit secondary antibody raised in a host species other than rabbit (eg donkey anti-rabbit secondary). Choosing a conjugateUnderstanding secondary antibodies - AbcamRecombinant Anti-PD-1 Antibody (HRP), Rabbit Monoclonal anti hectd1 rabbit primary antibodiesRecombinant Anti-PD-1 Rabbit Monoclonal Antibody (HRP) (50124-R010-H), manufactured by Sino Biological is validated in ELISA. Custom antibody services and bulk production also available. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here.Recombinant Anti-Rabbit IgG Fc Antibody [RMG02] (A121341)ELISA of IgGs from different species shows Anti-Rabbit IgG Fc Antibody [RMG02] reacts to the Fc region of rabbit IgG. There is no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Rabbit IgG Fc Antibody [RMG02] was used as the primary antibody.
Apr 01, 2018Anti-HECTD1 polyclonal antibody was purchased from Bethyl. Anti-ABCA1 monoclonal antibody was from Abcam. Anti-tubulin monoclonal, anti-myc polyclonal, secondary anti-mouse and anti-rabbit antibodies were from Sigma-Aldrich. Anti-ABCG1 polyclonal antibody was purchased from Novus Biologicals.Understanding secondary antibodies - Abcamgenerate the primary antibody. For example, if you use a primary antibody raised in . rabbit, you will need an anti-rabbit secondary antibody raised in a host species other than rabbit (eg donkey anti-rabbit secondary). Choosing a conjugate. Secondary antibodies tend to come in conjugated forms. For applications such asUnderstanding secondary antibodiesThe secondary antibody has to be directed against the isotype of the primary antibody. Polyclonal primary antibodies are generally raised in rabbit, goat, sheep or donkey and are an IgG isotype. The secondary antibody will typically be an anti-IgG H&L antibody. Monoclonal primary antibodies are commonly raised in mouse, rabbit and rat. For anti hectd1 rabbit primary antibodies
anti-CD117 (EP10) Rabbit Monoclonal Primary Antibody Deliver diagnostic confidence in evaluation of GIST. Roche has designed a new Class 1 IVD, anti-CD117 (EP10) Rabbit Monoclonal Primary Antibody reagent for immunohistochemistry testing. anti-CD117 (EP10) is a ready-to-use IHC antibody that enables you to evaluate the expression of CD117 protein with confidence.anti-HECTD1 antibody (FITC) Product No. ABIN2175483Rabbit Polyclonal HECTD1 antibody for IF (p). Order anti-HECTD1 antibody ABIN2175483.mouse anti-rabbit IgG-FITC Secondary Antibody SCBT anti hectd1 rabbit primary antibodiesOct 19, 2017If you have an interest in testing a 10 µg sample of mouse anti-rabbit IgG-FITC (sc-2359), our highly recommended monoclonal secondary antibody alternative to polyclonal anti-rabbit IgG-FITC secondary antibodies, please contact our Technical Service Department (or your local Distributor) for more information.
Apr 14, 2021mouse monoclonal secondary antibody conjugated to Horseradish Peroxidase (HRP) supplied at 200 µg in 0.5 ml volume. Strongly recommended alternative to polyclonal anti-rabbit IgG secondary antibodies for Enhanced Chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC) Highly specific secondary antibody that provides strong anti hectd1 rabbit primary antibodies