Anti-6X His tag&antibody (ab9108) AbcamJan 22, 2007All lanes Anti-6X His tag&antibody (ab9108) at 1/4000 dilution Lane 1 HeLa whole cell lysate - transfected with empty vector Lane 2 HeLa whole cell lysate - transfected with His-tagged gene Lysates/proteins at 10 µg per lane. Secondary All lanes HRP-conjugated goat anti-rabbit IgG ...
This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of What is his tag antibody?What is his tag antibody?Specificity / Sensitivity. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.His-Tag Antibody #2365 - Cell Signaling Technology
Posi-Tag is a ~45 kD recombinant protein that contains 12 different tags. It has a predicted weight of 45 kD and is intended for use as a positive control in western blot applications. The lysate contains the commonly used epitope tags HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, and FLAG tag.3/5(1)Category 6X His TagAnti-6X His tag antibody GTX115045 from GeneTex anti 6 x his tag rabbit polyclonal primary antibodiesThe Anti-6X His tag antibody was generated using 6X His tag antibody, His antibody, 6x His antibody, 6x his tag antibody, His Tag antibody, 6xHis Tag antibody, 6 x His antibody as the antigen. The Anti-6X His tag antibody from GeneTex is a Rabbit Polyclonal antibody to 6X His tag. The Anti-6X His tag antibody has been validated for the following Applications WB, IHC-Wm, IP, ELISA
Anti-6X His tag rabbit polyclonal antibody Applications ELISA, IP, WB Recommended Dilution WB 1:500-1:3000, IP:1:500, ELISA Target His tag Host Rabbit Clonality Polyclonal Immunogen Rabbit Polyclonal antibody to 6X His tag Isotype IgG Formulation 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Concentration lot specific Storage4/5Anti-His Tag Rabbit Polyclonal Antibody, HRP Conjugated anti 6 x his tag rabbit polyclonal primary antibodiesFeatured Anti-His Tag Rabbit Polyclonal Antibody, HRP Conjugated, specially designed for your Western blot analysis.,His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag,A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by anti 6 x his tag rabbit polyclonal primary antibodies6*his,His tag Antibody 10001-0-AP ProteintechExpressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
HRP Whole IgG Rabbit anti-6-His Tag Antibody HRP Conjugated suitable for WB, ICC, ELISA applications. Visit Bethyl for all your antibody needs.6x-His Tag Antibody (PA1-983B)The cells were labeled with 6x-His Tag Polyclonal Antibody (Product # PA1-983B) at 5 µg/mL and Histone H3 Monoclonal Antibody (865R2) (Product # AHO1432) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32733) and Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal Secondary Antibody, 6x-His Tag Rabbit anti-Tag, HRP, Polyclonal, Invitrogen anti 6 x his tag rabbit polyclonal primary antibodiesShop a large selection of Primary Antibodies - Alphabetical products and learn more about 6x-His Tag Rabbit anti-Tag, HRP, Polyclonal, Invitrogen 50 L; HRP Primary
Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. The Anti-6-His antibody has been used in western blotting. Physical formAnti-6X HIS EPITOPE TAG (RABBIT) Antibody - 600-401-3822 Western blot of Rabbit Anti-6xHIS Epitope Tag antibody.Lane NFR1-HIS recombinant protein.Load 50 ng per lane.Primary antibody - 1 NRF1 antibody at 1:1,000 overnight at 4°C.Primary antibody - 2 6xHIS Epitope tag antibody at 1:1,000 overnight at 4°C.Secondary antibody Peroxidase rabbit secondary antibody atAnti-6X His tag antibody (GTX115045) GeneTexThe HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Show More +. GTX115045 ELISA Image. 6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis.
Rabbit Polyclonal 6X His tag antibody. Validated in WB, ICC/IF, IP, ELISA. Cited in 2 reference(s).Anti-6X His tag antibody (HRP) GTX115045-01 from GeneTex anti 6 x his tag rabbit polyclonal primary antibodiesThe Anti-6X His tag antibody (HRP) from GeneTex is a Rabbit Polyclonal antibody. This antibody recognizes Epitope Tag antigen. The Anti-6X His tag antibody (HRP) has been validated for the following applications Western Blot.Anti-6X His tag&antibody (ab137839) AbcamImmunoprecipitation - Anti-6X His tag&antibody (ab137839) His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.
Jan 22, 2007All lanes Anti-6X His tag&antibody (ab9108) at 1/4000 dilution Lane 1 HeLa whole cell lysate - transfected with empty vector Lane 2 HeLa whole cell lysate - transfected with His-tagged gene Lysates/proteins at 10 µg per lane. Secondary All lanes HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution Developed using the ECL technique.Anti-6x His Tag Antibodies InvitrogenThe 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography.Anti-His Tag Antibody (monoclonal, 9C11)Anti-His Tag Antibody (monoclonal, 9C11) Boster Bio Anti-His Tag Antibody (monoclonal, 9C11) catalog # M30975. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species. Supplied as 100l/vial in Liquid form antibody. Cited in 6 publication(s).
His Tag antibody LS-C178368 is an unconjugated rabbit polyclonal antibody to His Tag. Validated for ELISA, IF, IHC, IP and WB.Anti-His Tag Antibody Rabbit Polyclonal ELISA,IHC,WB LSBioHis Tag antibody LS-C60077 is an unconjugated rabbit polyclonal antibody to His Tag. Validated for ELISA, IHC and WB. Cited in 1 publication.Anti-His Tag Rabbit Polyclonal Antibody - Abbkine anti 6 x his tag rabbit polyclonal primary antibodiesIt is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Alternative.
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (6 x His) Isotype. IgG2b. Formulation. PBS, pH 7.4 with 0.05% sodium azide. Concentration. lot specific. Storage. Store at -20°C.Anti-His tag polyclonal antibody (DPAB4057) - Creative anti 6 x his tag rabbit polyclonal primary antibodiesAnti-His tag antibody (DPAB4058) Custom Antibody Labeling We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor&dyes, DyLight&Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 g up to 1 g of IgG antibody.Anti-His tag polyclonal antibody (DPATB-H81678) - Creative anti 6 x his tag rabbit polyclonal primary antibodiesPyrroloquinoline quinone (PQQ) is an important redox active quinocofactor produced by a wide variety of bacteria. A key step in PQQ biosynthesis is a carbon-carbon cross-link reaction between glutamate and tyrosine side chains within the ribosomally synthesized peptide substrate PqqA. This reaction is catalyzed by the radical SAM enzyme PqqE.
IgGy Antibody Selector Quickly search hundreds of thousands of antibodies available for purchase from VWR by selecting common antibody features like antigen symbol and name, reactivity, clonality, conjugation, host, and other key factors. Antibodies used to identify and locate intracellular and extracellular proteins in common applications such as Western Blot, ELISA, ImmunoChemistry and anti 6 x his tag rabbit polyclonal primary antibodiesAnti-His-Tag antibody, Rabbit monoclonal - Sigma-AldrichAnti-His-Tag-Biotin antibody, Rabbit monoclonal. 1 Product Result Match Criteria Product Name, Property SAB5600192 ; recombinant, expressed in HEK 293 cells, clone RM146, purified immunoglobulin anti 6 x his tag rabbit polyclonal primary antibodies polyclonal WB 2 pricing. SAB1306085; polyclonal WB anti 6 x his tag rabbit polyclonal primary antibodiesAnti-Rad51 antibody, rabbit polyclonalAnti-Rad51 antibody, rabbit polyclonal 70-001 50 ul, 70-002 250 ul Storage Shipped at 4and stored at -20. anti 6 x his tag rabbit polyclonal primary antibodies Immunogen Full-size recombinant human Rad51 protein (His-tag removed) Form Rabbit antiserum added with 0.09% sodium azide. anti 6 x his tag rabbit polyclonal primary antibodies as the primary antibody and Alexa 594 labeled anti The lower Protocol for the use of anti-Rad51 anti 6 x his tag rabbit polyclonal primary antibodies
2 µL anti-s-tag rabbit polyclonal antibody and 15 l of 50 % Protein G agarose. Primary antibody Anti-s-tag rabbit polyclonal antibody,at 1:100 dilution . Secondary antibody Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution . Developed using the odssey technique. Performed under reducing conditions.Anti-TLR4 Antibody (SPC-200) Rabbit Polyclonal anti 6 x his tag rabbit polyclonal primary antibodiesFixation Formalin. Primary Antibody Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4°C. Secondary Antibody Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain Methyl Green at 200uL for 2 min at RT. Localization Inflammatory cells.Anti-V5 tag Antibody, Rabbit Polyclonal, 100378-T36 Sino anti 6 x his tag rabbit polyclonal primary antibodiesLane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate. Lane E:0.5 mg Negative control. 4 L anti-V5-tag rabbit polyclonal antibody and 60 g of Immunomagnetic beads Protein A/G. Primary antibody Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution.
Sensitivity and specificity of MonoRab TM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) to 6xHis-tag Fusion Protein by Western blot. Loading His tagged fusion protein Primary Antibody MonoRab TM Anti-His Tag Antibody (A01857-40) 0.2 g/ml Secondary Antibody Goat anti-rabbit IgG (H&L) [IRDye 800] (Licor,926-32211) 0.125 g/mlBioAcademia Low Prices & Free Shipping AntibodiesAnti-2S Globulin Antibody (A101697) 200µg / £295 11 Citations. Description Rabbit polyclonal antibody to 2S Globulin. Applications WB, IHC, Electron Microscopy, ELISA. Reactivity A thaliana 12S globulin (precursor and alpha subunit). Conjugate:Brand Sigma-Aldrich6-His Tag Antibody Bethyl Laboratories, Inc.Unconjugated Whole IgG Rabbit anti-6-His Tag Antibody Affinity Purified suitable for WB, IP, ICC, ELISA applications. Visit Bethyl for all your antibody needs.
The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2).Category Primary AntibodiesHistidine Tag and Anti-Histidine AntibodiesJan 24, 2021Abstract. A comprehensive review of the histidine tag and anti-polyhistidine antibodies including a summary of anti-polyhistidine antibodies cited among the over 60,000 formal publications in Labome's Validated Antibody Database. Histidine tag (His, polyhistidine) is one of most commonly used protein/tags for protein expression and purification.FtsH2/6/8 / Anti-Plant fts metallo-protease H2/6/8 antibodyArabidopsis Antibodies. View All Arabidopsis Antibodies; Chloroplast. View All Chloroplast; Photosynthesis I; Photosynthesis II; Cytochrome b6 / F complex; F-type ATPase; Antenna Proteins; Calvin cycle; Molecular Chaperone; Gene Transcription System; Protein Synthesis; Chloroplast TIC/TOC; Photosynthetic Electron Transport; Others; Mitochondria anti 6 x his tag rabbit polyclonal primary antibodies
His Tag Antibodies His Tag, also known as histidine tag or polyhistidine tag, is a common epitope tag used for protein purification and detection. A His Tag typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a protein.His Tag Antibodyanti His-Tag mAb-The best quality anti 6 x his tag rabbit polyclonal primary antibodiesI need a rabbit anti-His tag antibody which can recognize a His-tag fused in the internal site of a protein. I want to perform Flow Cytometry with this antibody. Which antibody should I use? A. Catalog No. A00174 is a rabbit anti-His tag antibody. It has been tested for internal His-tag recognition, but not for Flow Cytometry.His Tag antibody (FITC) anti His Tag antibody (FITC anti 6 x his tag rabbit polyclonal primary antibodiesHis Tag (FITC) Alternative Names. (click to expand) anti 6 His epitope tag antibody, anti Hexa His tag antibody, anti HHHHHH epitope tag antibody, anti HHHHHH tag antibody, anti His tag antibody, anti His tag 6X antibody. Product Properties. Form/Appearance. Liquid 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Concentration.
His tag 6X Alternative Names (click to expand) anti-6 His epitope tag antibody, anti-Hexa His tag antibody, anti-HHHHHH epitope tag antibody, anti-HHHHHH tag antibody, anti-His tag antibody, anti-His tag 6X antibodyHis-Tag (27E8) Mouse mAb Cell Signaling TechnologySpecificity / Sensitivity. His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with a His-Tag Antibody Cell Signaling TechnologyHis-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with a protein of unknown origin ~60-70kDa.
Rabbit. Clonality Polyclonal. Purification The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific). Specificity The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins. RRID AB_2837584NAD6 / Anti-NADH dehydrogenase 6 antibody - Arabidopsis Antibodies. View All Arabidopsis Antibodies; Chloroplast. View All Chloroplast; Photosynthesis I; Photosynthesis II; Cytochrome b6 / F complex; F-type ATPase; Antenna Proteins; Calvin cycle; Molecular Chaperone; Gene Transcription System; Protein Synthesis; Chloroplast TIC/TOC; Photosynthetic Electron Transport; Others; Mitochondria anti 6 x his tag rabbit polyclonal primary antibodiesPeople also askWhat is rabbit polyclonal IgG?What is rabbit polyclonal IgG?ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.Anti-6X His tag&antibody (ab9108) Abcam
In stock. Order within 17 hours and 20 mins to receive by Friday, April 23. Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-Ig (6*His, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L). Western blot of 6*His-tagged fusion protein with anti-6 anti 6 x his tag rabbit polyclonal primary antibodiesRabbit anti-His Tag antibody (ABIN1573881)Rabbit Anti-His-tag Polyclonal Antibody recognizes the His tag placed at N-terminal, C-terminal, and internal regions of targeted proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems.Some results are removed in response to a notice of local law requirement. For more information, please see here.
Home Products Antibody Primary Antibody anti- 6*his,His tag anti 6 x his tag rabbit polyclonal primary antibodies anti- 6*his,His tag antibody Catalogue No. FNab00010 Size 100µg Form liquid Purification Immunogen affinity purified Purity 95% as determined by SDS-PAGE Host Rabbit Clonality polyclonal Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20 anti 6 x his tag rabbit polyclonal primary antibodiesanti-6xHIS Tag Antibody (Rabbit) - HRP ConjugatedICL's polyclonal HRP conjugated rabbit anti-HIS tag antibody allows for specific detection of His-tagged fusion proteins. Please inquire for bulk pricing.